بررسی اثر متابولیت های تولیدی سویه بومی Pseudomonas sp UW4 بر بیان ژن p53 در رده سلولی SK-BR-3 سرطان پستان انسان

Introdution: Regarding to the development the problems of systemic toxicity and drug resistance in cancer chemotherapy, the continuing discovery of new bioactive compounds and anticancer agents is very necessary. Therefore, in this study, we checked the anticancer activity of native Pseudomonas sp. UW4 metabolite to find new compound. Methods: This experimental study was performed in Shahrekord Islamic Azad University from April 2015 to August 2015. First SK-BR-3 human breast cancer cells with different concentrations (5, 10 and 20 mg/ml) were treated with produced metabolite by native Pseudomonas sp. UW4 for 24 and 48 hours. Cell viability was assessed by MTS assay. Then, p53 expression was detected by RT-Real Time PCR. For statistical analysis, SPSS16 software and one way ANOVA test were used. Results: Treatment with produced metabolite by the native Pseudomonas sp. UW4 showed the decreases the viability of cells in a time and dose dependent manner, the most effective concentration of this substance was 20 mg/ml and 48 h after treatment. Also, an increase in p53 gene expression, significantly in 10 and  20 mg/ml after 24 h and 5, 10 and 20 mg/ml after 48h was observed (P<0.05). Conclusion: Produced metabolite by native Pseudomonas sp. UW4 could be used for treatment of SK-BR-3 breast cancer.